Coaches are replacing trains on the Seymour Line between Southern Cross and Seymour due to ongoing power outages between Kilmore East and Seymour.
Buses are now replacing trains on the Belgrave and Lilydale lines between Blackburn, Mooroolbark and Belgrave due to an equipment fault near Ringwood and a power issue near Upwey.
Transport Victoria has asked passengers to check station platform displays, listen for announcements, and allow an extra 45 minutes for their journey.
Coaches are also replacing trains on the Gippsland Line between Traralgon and Bairnsdale due to fallen trees and power outages.
A significant number of traffic lights remain affected across metropolitan Melbourne and regional Victoria due to widespread power outages.
At intersections where traffic lights are blacked out or flashing yellow, motorists should obey the rules of a Stop or Give Way sign.
The Western Highway is now closed to through traffic between Ballarat and Horsham due to fire activity. Local access is still available between Ballarat and Stawell only.
All other traffic between Ballarat and Horsham must detour via the Sunraysia Highway to St Arnaud and the Wimmera and Henty highways.
Other nearby closures include Murtoa-Glenorchy Road between Murtoa and Glenorchy, Stawell-Warracknabeal Road between Rupanyup and the Western Highway, Black Range Road between Stawell and Pomonal, and Ararat-Halls Gap Road between Moyston and Halls Gap.
The Ararat to Horsham coach service is diverting around these closures, adding up to 30 minutes to the journey.
Current emergency advice is available at emergency.vic.gov.au/